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新疆家蚕抗菌肽(Cecropin XJ)具有抑制肿瘤细胞生长的能力。为研究不同浓度Cecropin XJ体外对人胃癌细胞AGS生长的影响,分别采用MTT比色法、软琼脂集落形成实验、流式细胞术、划痕愈合和Transwell实验进行检测。结果表明,20~100μg/mL的Cecropin XJ能够显著抑制AGS细胞的增殖,并具有剂量和时间依赖性。同时,20,50,100μg/mL的Cecropin XJ处理后集落形成率分别降低了(35.81±13.10)%、(48.12±5.68)%和(81.46±6.21)%。AGS细胞经不同浓度Cecropin XJ处理24 h后,凋亡率逐渐上升并且细胞周期阻滞于S期。20μg/mL Cecropin XJ能够显著抑制AGS细胞的迁移和侵袭,100μg/mL Cecropin XJ几乎完全抑制AGS细胞的迁移和侵袭。以上结果说明,Cecropin XJ能够抑制AGS细胞生长、增殖,有可能成为人胃癌治疗的辅助药物。
The silkworm antibacterial peptide (Cecropin XJ) has the ability to inhibit the growth of tumor cells. In order to study the effects of different concentrations of Cecropin XJ on the growth of human gastric cancer cells in vitro, MTT colorimetric assay, soft agar colony formation assay, flow cytometry, scratch healing, and Transwell assay were used. The results showed that 20 ~ 100μg/mL Cecropin XJ can significantly inhibit the proliferation of AGS cells in a dose- and time-dependent manner. At the same time, the colony formation rate after treatment with 20, 50, 100 μg/mL Cecropin XJ was reduced by (35.81±13.10)%, (48.12±5.68)%, and (81.46±6.21)%, respectively. After AGS cells were treated with different concentrations of Cecropin XJ for 24 h, the apoptosis rate increased gradually and cell cycle arrested at S phase. 20μg/mL Cecropin XJ could significantly inhibit the migration and invasion of AGS cells. 100μg/mL Cecropin XJ almost completely inhibited the migration and invasion of AGS cells. The above results show that Cecropin XJ can inhibit the growth and proliferation of AGS cells and may become an adjuvant drug for the treatment of human gastric cancer.