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在批式试验中以牛粪堆肥为天然产氢菌源,分别以蔗糖和稀酸水解玉米秸秆为底物,通过厌氧发酵考察不同牛粪堆肥处理方式对发酵产氢性能的影响,并在此基础上构建了稀酸水解玉米秸秆两步发酵联产氢气-甲烷体系。当以蔗糖为底物时,牛粪堆肥处理方式对产氢影响不显著,产氢量均较高;当以稀酸水解玉米秸秆为底物时,室温浸泡处理的牛粪堆肥产氢效果最好,氢气最大产量达到213.8 m L/g TS。以稀酸水解玉米秸秆产氢发酵液为底物进行甲烷发酵,使秸秆的综合能量回收率达到41.2%,较单一发酵产氢时的能量回收率13.2%显著提高。
In the batch experiment, cow manure composting was used as the natural source of hydrogen producing bacteria. The effects of different manure composting modes on the hydrogen production were investigated by anaerobic fermentation using sucrose and dilute acid hydrolyzed corn stalks respectively. Based on this, a two-step fermentative co-production of hydrogen-methane system with dilute acid hydrolyzed corn stalks was established. When sucrose was used as substrate, the effect of cow dung compost treatment on hydrogen production was insignificant and the amount of hydrogen produced was high. When the acid hydrolyzed corn stalk was used as substrate, the hydrogen production efficiency of cow dung composting at room temperature Well, the maximum hydrogen production reached 213.8 m L / g TS. Methane fermentation was carried out using dilute acid hydrolyzed corn stalks to produce hydrogen fermentation broth as substrate, the comprehensive energy recovery rate of straw reached 41.2%, which was significantly higher than that of single fermentation hydrogen production.