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目的利用分子遗传学标记对采自云南西部楚雄、大理和怒江三地带绦虫进行生物多态性研究。方法取云南楚雄株、大理株、怒江株和贵州从江株成虫节片,抽提线粒体DNA,PCR扩增线粒体DNA细胞色素B(mtDNA-Cytb)序列部分区段,并测序;结合GenBank中已知猪带绦虫和亚洲带绦虫mtDNA-Cytb序列,构建系统发育树。结果序列分析显示云南大理和怒江带绦虫mtDNA-Cytb序列部分区段完全一致,同源性为100%;云南楚雄和贵州从江带绦虫mtDNA-Cytb序列部分区段基本一致,同源性为99%。系统发育树显示云南怒江和大理带绦虫与台湾亚洲带绦虫的遗传距离较近,距云南楚雄、贵州从江牛带绦虫较远,与猪带绦虫则更远。结论云南大理和怒江带绦虫属于亚洲带绦虫,云南楚雄带绦虫属于牛带绦虫。mtDNA-Cytb序列分析可以用于带绦虫生物多态性研究。
Objective To study the genetic diversity of tapeworms collected from three regions of Chuxiong, Dali and Nujiang in western Yunnan by molecular genetic markers. Methods Mitochondrial DNA was extracted from adult chinense of Chuxiong strain, Dali strain, Nujiang strain and Congjiang strain of Guizhou province. The mtDNA-Cytb fragment was amplified by PCR and sequenced. To know Taenia solium and Taenia asiatica mtDNA-Cytb sequence, the construction of phylogenetic tree. Results The sequence analysis showed that the mtDNA-Cytb sequences of Darya nigra and Nujiang nursery belonged to 100% homology. The mtDNA-Cytb sequences of Chuxiong Yunnan and Taichongjiang of Guizhou were basically the same with a homology of 99 %. Phylogenetic tree showed that the genetic distance between Nujiang and Dali Tape Yunnan was close to that of Taiwan Taenia saginata, and far from Taenia saginata in Yunnan, but also to Taenia saginatai in Congjiang, Guizhou. Conclusion The tapeworms of Dali and Nujiang belonged to Taenia saginata and Taenia sapiens of Yunnan belonged to Taenia saginata. mtDNA-Cytb sequence analysis can be used to study TaeniaBs polymorphism.