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目的:检测乳腺癌患者血浆循环DNA含量,探讨其与临床病理特征之间的关系。方法:收集80例乳腺癌、40例乳腺良性病变患者和97例健康自愿者的外周血。用微量基因组DNA提取试剂盒提取血浆DNA,用荧光染料试剂盒和QubitTM荧光仪测定血浆DNA含量。结果:(1)血浆循环DNA含量在组织学分级Ⅰ级和Ⅱ～Ⅲ级、患者肿瘤直径>20mm和≤20mm之间有统计学差异(P<0.05);而在淋巴结转移阴性和阳性、雌激素受体阴性和阳性之间均无统计学意义。(2)乳腺癌组血浆循环DNA浓度明显高于乳腺良性病变组和正常对照组,相比较有统计学差异(P<0.05);而良性病变组血浆循环DNA浓度高于正常对照组,但差异无统计学意义。(3)以血浆循环DNA≤12.90μg/L作为诊断乳腺癌的临界值,其检测灵敏度为85.0%,特异度为88.3%,准确度为87.1%。(4)Roc曲线下面积(AUC)为0.879,95%可信区间为0.830～0.928。结论:乳腺癌患者的血浆DNA水平与疾病的发生、发展和预后密切相关,血浆DNA水平有助于乳腺癌的鉴别诊断。
Objective: To detect circulating DNA content in plasma of patients with breast cancer and to explore its relationship with clinicopathological features. Methods: Peripheral blood samples from 80 patients with breast cancer, 40 patients with benign breast disease and 97 healthy volunteers were collected. Plasma DNA was extracted using a mini-genomic DNA extraction kit and plasma DNA content was measured using a fluorescent dye kit and a Qubit ™ fluorometer. Results: (1) There was a significant difference in the levels of circulating DNA in histological grades Ⅰ and Ⅱ ~ Ⅲ between patients with tumor diameter> 20mm and ≤20mm (P <0.05). However, in patients with lymph node metastasis negative and positive Hormone receptor negative and positive between no statistically significant. (2) The plasma DNA concentration in breast cancer group was significantly higher than that in benign breast disease group and normal control group (P <0.05), while the circulating DNA concentration in benign lesion group was higher than that in normal control group No statistical significance. (3) The detection rate of plasma DNA ≥12.90μg / L for breast cancer was 85.0%, the specificity was 88.3%, and the accuracy was 87.1%. (4) The area under the curve of Roc (AUC) was 0.879, 95% confidence interval was 0.830 ~ 0.928. Conclusion: Plasma DNA level in patients with breast cancer is closely related to the occurrence, development and prognosis of the disease. Plasma DNA level is helpful for the differential diagnosis of breast cancer.