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本文采用PCR-SSCP和测序组合技术检测了6个产地甘草的β-香树脂醇合成酶(β-AS)基因的SNP,并进行了SNP和产地的相关性分析,探索甘草道地性形成的分子机制。结果显示,甘草β-AS基因第一外显子存在1处SNP,可将甘草划分为94A型、94G型和94A/G型3种基因型,其中94A型在道地产区所占比例要远远高于非道地产区,达到极显著差异(P<0.001)。本文研究证明,甘草β-AS基因94位点的SNP可能是导致甘草道地性形成的机制之一。
In this paper, PCR-SSCP and sequencing techniques were used to detect the SNP of β-amyrin synthase (β-AS) gene in six licorice producing areas, and the correlation analysis between SNP and origin was carried out to explore the licorice Molecular mechanism. The results showed that there was one SNP in the first exon of β-AS gene of licorice, which could be divided into 94A, 94G and 94A / G genotypes, among which 94A genotype had a far lower proportion Far higher than non-road real estate area, reaching a significant difference (P <0.001). This study shows that the SNP at position 94 of the glycogen β-AS gene may be one of the mechanisms leading to the licorice formation.