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目的探讨胸腺肽(thymosinαl,Tα1)对促进人脐静脉血管内皮细胞(human umbilical veinvessel endothelial cells,HUVECs)增殖的影响以及刺激血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的合成情况。方法体外培养HUVECs细胞系,采用四甲基偶氮唑蓝法(MTT)法测定HUVECs的增殖情况,以流式细胞仪检测细胞周期,Western blot检测VEGF在HUVECs的合成。结果浓度为2.5、5、10μg/ml的Tα1作用于HUVECs后,其对应的D(492)值、增殖率分别为(0.498±0.027)、(96.2±4.5)%,(0.737±0.018)、(182.0±5.8)%,(0.745±0.021)、(185.0±6.2)%与对照组比较,明显增高(P<0.01)。S期细胞增多。Western blot检测结果显示实验组VEGF条带灰度值/内参灰度值(0.29±0.01)与对照组(0.12±0.06)相比,显著上升(P<0.05)。结论Tα1能促进HUVECs的增殖及增强血管内皮细胞内VEGF的合成和分泌,在创伤组织修复过程中可能发挥促新生血管形成的作用。
Objective To investigate the effects of thymosin α1 (Tα1) on the proliferation of human umbilical vein vascular endothelial cells (HUVECs) and to stimulate the synthesis of vascular endothelial growth factor (VEGF). Methods HUVECs were cultured in vitro. The proliferation of HUVECs was detected by MTT method. The cell cycle was detected by flow cytometry. The VEGF synthesis in HUVECs was detected by Western blot. Results The corresponding D (492) values and proliferation rates of Tα1 with concentration of 2.5, 5 and 10 μg / ml were (0.498 ± 0.027), (96.2 ± 4.5)%, (0.737 ± 0.018) and 182.0 ± 5.8)%, (0.745 ± 0.021) and (185.0 ± 6.2)%, respectively, were significantly higher than those in the control group (P <0.01). S phase cells increased. The result of Western blot showed that the grayscale value of gray matter / gray value of internal standard (0.29 ± 0.01) in experimental group was significantly higher than that in control group (0.12 ± 0.06) (P <0.05). Conclusion Tα1 can promote the proliferation of HUVECs and enhance the synthesis and secretion of VEGF in vascular endothelial cells. It may play an important role in angiogenesis during wound repair.