通过单因子和正交试验对玉米弯孢菌叶斑病[Curvularialunata(Waker)]拮抗放线菌BPS2进行了发酵条件的研究,包括发酵液的初步筛选、碳源、氮源、初始pH值、装液量、发酵时间等。结果表明,最佳培养基和培养条件为1000mL培养基中蔗糖2.0%、玉米粉2.0%、黄豆饼粉3.0%、蛋白胨0.6%、硫酸铵0.3%、磷酸二氢钾0.1%、氯化钠0.1%、碳酸钙0.3%,培养初始pH值为6,250mL三角瓶装液量为30mL,发酵周期为60h。 The fermentation conditions of antagonistic actinomycete BPS2 against Curvularialunata (Waker) were studied by single factor and orthogonal test, including preliminary screening of fermentation broth, carbon source, nitrogen source, initial pH value, Liquid volume, fermentation time. The results showed that the best medium and culture conditions were 2.0% sucrose, 2.0% corn flour, 3.0% soybean meal, 0.6% peptone, 0.3% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.1% sodium chloride 0.1% %, Calcium carbonate 0.3%, the initial pH of culture 6,250mL flask volume 30mL, the fermentation cycle was 60h.