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目的:建立复方中风康复片中士的宁和马钱子碱的质量分数测定方法。方法:采用高效液相色谱法,以ODSC18(4.6 mm×250 mm,5μm)为分离柱,以乙腈-0.1%磷酸与0.1%三乙胺等量混合溶液(12∶88)为流动相,260 nm为检测波长,柱温为室温。结果:士的宁和马钱子碱的线性范围分别为0.025～0.303μg和0.012～0.200μg,平均回收率分别为96.6%和96.4%,RSD分别为0.77%和0.73%(n=6)。结论:该方法简便、准确、重复性好,可作为该制剂含量测定方法。
OBJECTIVE: To establish a method for the determination of strychnine and strychnine in the compound stroke rehabilitation tablets. Methods: High performance liquid chromatography (HPLC) was used with ODSC18 (4.6 mm × 250 mm, 5 μm) as the separation column and a mobile phase of acetonitrile-0.1% phosphoric acid and 0.1% nm is the detection wavelength, the column temperature is room temperature. Results: The linear ranges of strychnine and brucine were 0.025 ~ 0.303μg and 0.012 ~ 0.200μg, the average recoveries were 96.6% and 96.4%, respectively. The RSDs were 0.77% and 0.73%, respectively (n = 6). Conclusion: The method is simple, accurate and reproducible, and can be used as a method for determination of the content of the preparation.