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目的:研究糖酵解途径关键酶磷酸果糖激酶1(PFK1)的蛋白及其酶活性在鼻咽癌活检组织中的表达情况,并探讨其意义。方法:采取病例-对照设计的方法 ,实验组为41份鼻咽癌患者的鼻咽癌活检组织标本,对照组为20份鼻咽慢性炎性患者的鼻咽部活检组织标本,通过Western blot检测PFK1蛋白的表达;酶学分析的方法检测PFK1酶活性。结果:实验组的PFK1蛋白表达及其酶活性水平均明显高于对照组(P<0.01);在实验组中,伴有淋巴结转移的鼻咽癌活检标本中的PFK1蛋白及其酶活性表达水平均明显高于无淋巴结转移的鼻咽癌活检标本(P<0.01)。结论:PFK1与鼻咽癌的发生、发展密切相关,可能作为鼻咽癌的肿瘤标志物之一。
OBJECTIVE: To study the expression of PFK1 protein and its enzymatic activity in nasopharyngeal carcinoma biopsies and to explore its significance. Methods: A case-control study was conducted in 41 nasopharyngeal carcinoma biopsy specimens in the experimental group and 20 nasopharyngeal biopsy specimens from chronic inflammatory patients with nasopharyngeal lesions in the control group. Western blot analysis PFK1 protein expression; enzymatic analysis method to detect PFK1 enzyme activity. Results: The levels of PFK1 protein and its activity in the experimental group were significantly higher than those in the control group (P <0.01). In the experimental group, the expression level of PFK1 protein and its activity in nasopharyngeal carcinoma biopsy specimens with lymph node metastasis Were significantly higher than those without lymph node metastasis of nasopharyngeal biopsy specimens (P <0.01). Conclusion: PFK1 is closely related to the occurrence and development of nasopharyngeal carcinoma and may be one of the tumor markers of nasopharyngeal carcinoma.