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目的动态观察低剂量砷染毒小鼠致肝脏脂质过氧化情况。方法96只健康雄性KM小鼠随机分组,设0、50、500和5000μg/L4个组,再将每个剂量组分4、6和8周3个染毒时间点,经饮水染毒,测定肝脏和血液中总砷含量、肝组织脂质过氧化和抗氧化水平。结果血砷、肝脏砷含量随着染毒时间和染毒剂量的增加而逐渐增高,差异有统计学意义(P<0.05)。在肝组织中,丙二醛(MDA)含量均随染毒时间和染毒剂量的增加而增高,差异有统计学意义(P<0.05);过氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)活性则是均随着染毒时间和染毒剂量的增加出现先增高后降低的现象,其增高差异均有统计学意义(P<0.05)。GSH-Px活性降低与对照组之间,差异有统计学意义(P<0.05),而SOD活性降低与对照组相比无明显差异(P>0.05)。结论在本试验条件下,低砷可以诱导小鼠肝脏抗氧化损伤系统出现适应性的增高,但随着剂量和作用时间增加最终会造成小鼠的氧化损伤。
Objective To observe the liver lipid peroxidation caused by low dose arsenic in mice. Methods 96 healthy male KM mice were randomly divided into four groups: 0, 50, 500 and 5000 μg / L, and each dose component was exposed to drinking water at 4, 6 and 8 weeks after exposure to drinking water Total arsenic levels in liver and blood, lipid peroxidation and antioxidant levels in liver tissue. Results The contents of arsenic and arsenic in liver increased gradually with the increase of exposure time and dosage, the difference was statistically significant (P <0.05). In the liver tissue, malondialdehyde (MDA) content increased with the increase of exposure time and dose, the difference was statistically significant (P <0.05); the activity of superoxide dismutase (SOD) and glutathione Peptides peroxidase (GSH-Px) activity increased first and then decreased with the increase of exposure time and dose, and the difference was statistically significant (P <0.05). The activity of GSH-Px in the control group was significantly lower than that in the control group (P <0.05), while the activity of SOD was not significantly different from that in the control group (P> 0.05). Conclusions Under this test condition, low arsenic can induce adaptive increase of anti-oxidative injury system in mouse liver, but eventually lead to oxidative damage in mice with dose and duration of action.