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目的:建立薄层色谱法测定薏苡种皮糠中氨基酸含量的方法。方法:采用硅胶G薄层板,展开剂为正丁醇-无水乙醇-冰醋酸-水(8∶2∶2∶3),茚三酮试液显色,双波长锯齿扫描,λS=550nm,λR=710nm。结果:缬氨酸线性范围为0.096~0.672μg(r=1.000),平均回收率为101.80%,RSD=2.33%;亮氨酸线性范围为0.1832~1.2824μg(r=0.996),平均回收率为97.97%,RSD=2.13%;丙氨酸线性范围为0.3224~2.3268(r=0.996),平均回收率为102.37%,RSD=2.17%。结论:方法用于氨基酸的测定简便,快速,准确。
Objective: To establish a method for the determination of amino acids in Coix seed coat skin by TLC. Methods: Using silica gel G plate, developing solvent was n-butanol-ethanol-glacial acetic acid-water (8:2:2:3) , λR = 710 nm. Results: The linear range of valine was 0.096 ~ 0.672μg (r = 1.000), the average recovery was 101.80%, RSD was 2.33%. The linear range of leucine was 0.1832 ~ 1.2824μg (r = 0.996) 97.97%, RSD = 2.13%. The linear range of alanine was 0.3224 ~ 2.3268 (r = 0.996). The average recovery was 102.37% and RSD was 2.17%. Conclusion: The method for the determination of amino acids is simple, rapid and accurate.