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目的微血管单元是营养物质运输及血流动力学行为调控的关键场所,但体内微血管单元的研究常受限于伦理学、经济及技术等问题,构建体外微血管网络模型将有助于微血管相关病理生理行为的研究。方法该文以正常人眼底微动脉网络结构为模型,设计含有体内微血管网络特征的微血管单元模型;利用软光刻加工技术在体外构建出微血管网络模型,该模型具有不对称网络结构及微血管网络的分叉和侧支结构;在该体外模型上量化模型内微通道中细胞耗尽层(CDL)厚度以及红细胞分配。结果与结论所构建的体外微血管网络模型能够揭示体内微血管单元的血流动力学重要特征,包括CDL厚度、血细胞容积率和红细胞分配间的相互关系。该研究将为体外研究微血管内血流动力学及微血管系统疾病提供一种新的思路及技术手段。
Microvascular unit of interest is a key site for the regulation of nutrient transport and hemodynamic behavior. However, the study of microvascular units in vivo is often limited to ethical, economic and technical issues. Establishing an in vitro microvascular network model will contribute to microvascular-associated pathophysiology Behavior research. Methods In this study, the model of capillary network of human fundus arteriole was used as a model to design a microvascular unit model with in vivo microvascular network. A microvascular network model was constructed by soft lithography in vitro. The model has asymmetric network structure and microvascular network Bifurcation and collateral structures; on the in vitro model, the thickness of the cell depletion layer (CDL) in the microchannels within the model and the red blood cell distribution were quantified. RESULTS AND CONCLUSION The constructed in vitro microvascular network model reveals important hemodynamic characteristics of in vivo microvascular units, including the relationship between CDL thickness, hematocrit rate, and erythrocyte partitioning. This study will provide a new way of thinking and technical means for the study of microvascular hemodynamics and microvascular diseases in vitro.