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本文采用了修改的ANAE法,对正常人外周血涂片进行孵育,观察用α—醋酸萘酯为底物时改变孵育液pH、孵育温度和孵育时间等条件对显示淋巴细胞ANAE活性的影响。结果表明,孵育液pH为5.8时淋巴细胞阳性率最高,适宜pH范围为pH5.8±0.3。孵育温度在20°～37℃之间均可采用,但ANAE阳性率随孵育时间的延长而升高,升高的速度和幅度与孵育温度有关。在20℃孵育4～6小时和37℃孵育3±0.5小时,淋巴细胞ANAE阳性率在70％左右,与一般所认为的正常值一致。未固定涂片在室温下干燥保存30天,ANAE活性不受影响,因而标本可以留存集中做或邮寄托做。本文对ANAE鉴别淋巴细胞亚群的可靠性及ANAE反应类型与淋巴细胞亚群的关系进行了讨论。
In this paper, a modified ANAE method was used to incubate normal human peripheral blood smear and observe the effect of varying pH, incubation temperature and incubation time on the activity of ANAE in lymphocytes when using α-naphthyl acetate as a substrate. The results showed that the positive rate of lymphocyte was the highest when the pH was 5.8, and the suitable pH range was pH 5.8 ± 0.3. Incubation temperature between 20 ° ~ 37 ℃ can be used, but the positive rate of ANAE increased with the incubation time, the speed and magnitude of the increase and incubation temperature. Incubation at 20 ℃ for 4 ~ 6 hours and 37 ℃ for 3 ± 0.5 hours, the positive rate of ANAE of lymphocytes is about 70%, which is consistent with the generally accepted normal value. Unfixed smears were stored at room temperature for 30 days, and the activity of ANAE was not affected. Therefore, specimens could be left undisturbed or centrally placed. This article discusses the reliability of ANAE in identifying lymphocyte subsets and the relationship between ANAE response types and lymphocyte subsets.