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采用水蒸汽蒸馏-乙醚同时萃取装置对抗白背飞虱品种RathuHeenati(RHT),IR64,Nabeshi和感虫对照品种TN1稻株进行挥发性次生物质提取.浓缩所得精油,经“气谱-质谱”(GC-MS)联机检测出64个有机的化学组分,其中36种主要分子结构为:9种醇.9种醛.7种酮4种酯,3种烷烃.2种酸,烯烃和杂环化合物各1种。2-已烯醛、β-紫罗兰酮、叶醇(反式)、叶绿醇、3,5.5-三甲基-3-环已烯-1-酮和水杨酸甲酯的含量较高。将蒸馏提取物回喷在TN1稻株上,白背飞虱对抗虫和感虫稻株蒸馏提取物的趋性没有显著差异。嗅觉仪测定飞虱的趋性结果表明。单体化合物苯甲醇和已烯醛比对照丙酮更易吸引白背飞虱。在回喷TN1蒸馏提取物、丙酮溶液和未处理的TN1稻株上,白背飞虱分泌的蜜露量明显地较在Nabeshi和IR64蒸馏提取物处理过的TN1稻株上分泌的高。白背飞虱从掺有蒸馏提取物和挥发物单体的营养液中吸食的量均比从单纯营养液中的少;抗虫品种RHT蒸馏提取物对白背飞虱拒食作用最强,β-紫罗兰酮和已烯醛次之。
A steam distillation - ether simultaneous extraction device was used to extract volatile secondary metabolites from RathuHeenati (RHT), IR64, Nabeshi and the susceptible control strain TN1 of whitebacked planthopper. The resulting essential oil was concentrated and 64 organic chemistries were detected by gas chromatography-mass spectrometry (GC-MS) with 36 of the main molecular structures being nine alcohols. Nine kinds of aldehydes. 7 ketones 4 esters, 3 alkanes. Two kinds of acid, one kind of alkene and heterocyclic compound. 2-hexenal, β-ionone, phytol (trans), phytol, 3,5,5-trimethyl-3-cyclohexen-1-one and methyl salicylate high. The distillation extract was sprayed back on TN1 rice plants, and the repellency of the whitebacked planthopper against insect pest and the susceptible rice plant distillation extract was not significantly different. Odor instrument to measure the tendency of planthopper results show. Monomeric compounds benzyl alcohol and hexenoic acid are more likely to attract whitebacked planthopper than control acetone. The amount of nectar secreted by BPPH was significantly higher than that of TN1 treated with Nabeshi and IR64 distilled extracts on spray TN1 distilled extract, acetone solution and untreated TN1 rice plants. The amount of white-backed planthopper feeding from nutrient solution mixed with distilled and volatile monomer was less than that from pure nutrient solution. Anti-insect RHT distillate extract had the strongest antifeeding effect on white-backed planthopper, Ionone and hexenal are second to none.