论文部分内容阅读
利用5′RACE试剂盒对从中国不同地区、不同SARS患者体中分离的SARS-CoV基因组5′端序列进行RT-PCR扩增,并将扩增产物克隆至T easy vector。扩增片段的序列测定结果表明:所分离的4株SARS-CoV基因组5′端非编码区的核苷酸序列和其他国家和地区报道的序列基本一致,而且所形成二级结构也完全相同,但与已知普通冠状病毒的差别较大。同时发现在依赖于RNA的RNA聚合酶起始密码子上游-197 nt处有冠状病毒典型的转录调控核心保守序列5′-CUAAAC-3′。
The 5’RACE kit was used to amplify the 5’-end sequence of SARS-CoV genome isolated from different SARS patients in different regions in China and the amplified product was cloned into T easy vector. Sequence analysis of the amplified fragment showed that the nucleotide sequences of the 5 ’untranslated regions of the 4 isolates of SARS-CoV isolated from the other isolates were basically the same as those reported in other countries and regions, and the formed secondary structures were identical. But with the known difference between the common coronavirus. At the same time, it was found that there is a conserved core sequence 5’-CUAAAC-3 ’of the typical transcriptional regulatory coronavirus located at -197 nt upstream of the RNA-dependent RNA polymerase initiation codon.