论文部分内容阅读
首次对台湾线虫草无性型—黄山被毛孢菌株的交配型基因进行了较为系统的研究。采用稀释涂布平板法和显微定位法获得台湾线虫草的单次生子囊孢子,并对单次生子囊孢子菌株的交配型基因进行PCR扩增和子实体人工诱导实验。结果表明,在34株单次生子囊孢子菌株中,14个菌株含有MAT1-1基因,4个菌株含有MAT1-2基因,其余16个菌株同时含有MAT1-1和MAT1-2基因;人工诱导子实体的结果显示,含有2种交配型基因的菌株可以生长出更多的子实体。提示台湾线虫草的交配类型可能为同宗配合。
For the first time, we did a systematic study on the mating type genes of Cordyceps militaris in Taiwan. The single ascospore of Cordyceps militaris was obtained by the method of dilution coating and microdisposition. The mating type gene of single ascosporogenous strain was amplified by PCR and artificially induced by fruiting body. The results showed that among the 34 single-stage ascosporogenous strains, 14 strains contained MAT1-1 gene, 4 strains contained MAT1-2 gene, and the other 16 strains contained both MAT1-1 and MAT1-2 genes. The artificial inducer The results of the entity show that strains containing two mating type genes can grow more fruiting bodies. Tip Cordyceps Taiwan mating type may be the same line with.