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提出一种基于81°倾斜光纤光栅(81°-TFG)的新城疫病毒(NDV)免疫传感器。分析81°-TFG传感器的基本原理和传感特性。利用金黄色葡萄球菌蛋白A(SPA)修饰81°-TFG的表面,然后将自制的高纯度新城疫病毒单克隆抗体(MAbs)通过SPA分子固定于81°-TFG表面,从而制成对NDV抗原特异性检测的传感器。实验结果表明:该免疫传感器对NDV的探测极限在0.1~0.2ng/mL之间,检测饱和点约为1.0ng/mL;在0~1.0ng/mL范围内具有良好的线性度(R2约为0.982),灵敏度约为342pm/(ng·mL-1);且该传感器具有良好的可重用性和对NDV抗原的高度特异性,并能用于NDV的临床检测。相对于传统的免疫荧光技术、酶联免疫等生化检测方法,本文提出的方法具有免标记、操作简便、快速检测等优点。
A Newcastle Disease Virus (NDV) immunosensor based on an 81 ° -oriented fiber grating (81 ° -TFG) was proposed. Analyze the basic principles and sensing characteristics of 81 ° -TFG sensors. The surface of 81 ° -TFG was modified with Staphylococcus aureus protein A (SPA), and the self-made high-purity Newcastle Disease Virus monoclonal antibody (MAbs) was immobilized on the surface of 81 ° -FGFG through SPA molecules to prepare the NDV antigen Specific detection of the sensor. The experimental results show that the detection limit of the immunosensor for NDV is between 0.1-0.2ng / mL and the detection saturation point is about 1.0ng / mL. The calibration curve of the immunosensor has good linearity in the range of 0-1.0ng / mL 0.982), the sensitivity is about 342pm / (ng · mL-1); And the sensor has good reusability and high specificity for NDV antigen, and can be used for NDV clinical testing. Compared with the traditional immunofluorescence technology, enzyme-linked immunosorbent assay and other biochemical methods, the proposed method has the advantages of no mark, easy operation and rapid detection.