论文部分内容阅读
目的家蝇是一种广泛分布的重要公共卫生害虫,开展其遗传学、分子生物学的研究为认识和利用家蝇,有效地控制蝇传疾病提供科学依据。方法检测我国5个家蝇种群中拟除虫菊酯类杀虫剂抗性等位基因的频率,通过分析在无杀虫剂选择条件下抗性等位基因频率的变化,推测抗性等位基因适合度效应。结果研究数据显示,与拟除虫菊酯类代谢抗性相关联的细胞色素P450(CYP6D1v1)抗性等位基因在广东、上海和北京家蝇种群中频率上升,表明CYP6D1v1抗性等位基因不存在适合度代价;而CYP6D1v1等位基因在起始频率很低的山东和吉林种群中消失,可能是遗传漂变的结果。家蝇钠离子通道(Vssc)抗性等位基因(kdr-his)频率在广东、山东和吉林种群中下降,而在上海和北京种群中则有所上升,其中变化最大的是山东种群,kdr-his基因频率由起始较高的0.33降至0,表明kdr-his等位基因的适合度因种群的不同而不同。进一步的序列分析表明,kdr-his等位基因可以区分为不同的单倍型,上述kdr-his基因频率的变化因种群而异,可能是因为不同家蝇种群其钠离子通道基因单倍型有所不同。结论不同的抗性基因或相同基因的不同单倍型具有不同的适合度,提示家蝇抗药性检测和抗性治理要因地制宜。
Purpose Housefly is a widely distributed important public health pest, carry out its genetics, molecular biology research for understanding and use of housefly, fly fly control disease effectively provide the scientific basis. Methods The frequency of the pyrethroid insecticide resistance alleles in five Musca domestica populations was tested. By analyzing the changes of the allele frequencies of the alleles without pesticide selection, we speculated that the alleles of the resistance alleles were suitable Degree effect. Results The data show that the frequency of cytochrome P450 (CYP6D1v1) resistance alleles associated with pyrethroid-type metabolic resistance increases in Musca domestica population in Guangdong, Shanghai and Beijing, suggesting that the CYP6D1v1 resistance allele is not suitable While the allele of CYP6D1v1 disappeared in Shandong and Jilin populations with low initial frequency, which may be the result of genetic drift. The frequency of kdr-his allele in housefly decreased in Guangdong, Shandong and Jilin populations, but increased in Shanghai and Beijing populations. The most significant change was kr The -his gene frequency decreased from a high of 0.33 to 0, indicating that the fitness of the kdr-his allele varies from population to population. Further sequence analysis showed that the kdr-his allele could be divided into different haplotypes. The frequency of kdr-his gene changes may vary from species to species, probably because the haplogroups of sodium ion channel genes Different. Conclusion The different haplotypes of different resistance genes or the same genes have different fitness, suggesting that the detection of Musca domestica resistance and resistance management should be tailored to local conditions.