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实验发现,阿奇霉素对联吡啶钌的电致化学发光(ECL)具有显著的增强作用。据此,建立了以金电极为工作电极的测定阿奇霉素ECL分析新方法。采用了循环伏安(CV)和ECL法,研究了阿奇霉素对联吡啶钌体系的电化学行为和ECL行为的增强作用。结果表明,在最佳条件下,阿奇霉素浓度在2.0×10-4～4.0×10-7 mol/L范围内与相对发光强度呈线性关系,其线性回归方程为I计数=22.848×106 C+221.8(r=0.9981,n=12)。检出限为3.00×10-9 mol/L(S/N=3)。连续平行测定1.0×10-5 mol/L的阿奇霉素标品溶液10次,发光强度值的相对标准偏差(RSD)为2.09%。对样品进行回收率试验,回收率在95.0%～102.0%之间,RSD为2.40%(n=5)。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于药物中阿奇霉素的测定,结果满意。
It was found that azithromycin can significantly enhance the electrochemiluminescence (ECL) of bipyridyl ruthenium. Based on this, a new method for the determination of azithromycin ECL using gold electrode as working electrode was established. Cyclic voltammetry (CV) and ECL method were used to study the electrochemical behavior of azithromycin and the enhancement of ECL behavior. The results showed that under the optimal conditions, the concentration of azithromycin was linear with the relative luminescence intensity in the range of 2.0 × 10-4 ~ 4.0 × 10-7 mol / L. The linear regression equation was I = 22.848 × 106 C + 221.8 (r = 0.9981, n = 12). The detection limit was 3.00 × 10-9 mol / L (S / N = 3). The relative standard deviations (RSDs) of luminescence intensity were 2.09% for 10 consecutive times of 1.0 × 10-5 mol / L azithromycin standard solution. The recoveries of the samples were between 95.0% and 102.0% with a RSD of 2.40% (n = 5). The method has high selectivity and sensitivity, sample processing is simple and fast, for the determination of azithromycin in the drug, the results are satisfactory.