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目的 了解重型乙型肝炎( 乙肝) 病人血清乙肝病毒(HBV)DNAC基因启动子(CP) 的变异。方法 对用聚合酶链反应(PCR) 法扩增的血清HBVDNA 直接测序。结果 7 例亚急性重型肝炎病人的HBV 分离株CP区分别有2~12 个替代变异,1 例病人有11bp 的碱基插入。CP变异主要发生于CP的第1 和第2 个AT丰富区,nt1 762 和nt1 764 的替代变异见于7 例亚急性重型肝炎病人的4 例中,是CP变异的热点,其中3 例HBeAg 阴性,说明和HBeAg 阴性表型相关。CP的第3 个AT丰富区、HBV逆转录起始位点(DR1) 和前C基因、前基因组转录起始位点未见变异。结论 重型肝炎病人的HBVCP区存在较多的变异,CP变异主要发生于和前C基因相关的第1 和第2 个AT丰富区,可能和HBeAg 阴性表型相关
Objective To understand the variation of DNAC gene promoter (CP) of hepatitis B virus (HBV) in patients with severe hepatitis B (hepatitis B). Methods Serum HBVDNA amplified by polymerase chain reaction (PCR) was directly sequenced. Results Seven to seven substitution mutations were found in the CP region of HBV isolates in seven patients with subacute severe hepatitis and one in 11 bp. CP mutation occurred mainly in the first and second AT rich region of CP, and the substitution mutation of nt1 762 and nt1 764 was found in 7 subacute severe hepatitis patients in 4 cases, which was the hot point of CP mutation. Among them, 3 cases were negative for HBeAg, Description and HBeAg negative phenotype related. The third AT-rich region of CP, HBV reverse transcription initiation site (DR1) and pre-C gene, the former genome transcription initiation site no mutation. Conclusion There are many variations in the HBVCP region of patients with severe hepatitis. The variation of CP mainly occurs in the first and second AT-rich regions associated with the pre-C gene, which may be related to the negative phenotype of HBeAg