综述了近几年来文献报导的适用于生物学研究的富勒烯类物质的制备方法。这些方法可以归纳为两类:(1)利用特定化合物的介导作用将C_(60)分子溶在水中,包括四氢呋喃-丙酮介导法、Triton X-100介导法、聚乙烯基吡咯烷酮介导法、环糊精包埋法和脂质体包埋法;(2)合成水溶性的C_(60)衍生物,包括多羟基衍生物、羧酸衍生物与带各种生物活性基团的衍生物等。文章还对各种制备方法的应用进行了简要讨论。 The preparation methods of fullerenes suitable for biological research reported in the literature in recent years are reviewed. These methods can be summarized into two categories: (1) C 60 molecules are dissolved in water using the mediation of specific compounds, including tetrahydrofuran-acetone mediated, Triton X-100 mediated, polyvinylpyrrolidone mediated (2) synthesis of water-soluble C 60 derivatives, including polyhydroxy derivatives, carboxylic acid derivatives and derivatives with a variety of bioactive groups And so on. The article also briefly discusses the application of various preparation methods.