水稻的转基因研究一般以潮霉素磷酸转移酶(HyglomychlPhosphtobamfemse,6P6)基因HPT作为选择标记,因此潮霉素抗性检测的可靠性至关重要。该项研究以中花11和日本晴的T1,T2和T6代转基因水稻为材料,研究了苗期、减数分裂的完全伸展叶和乳熟期不同部位的叶片对潮霉素的抗性反应,同步提取相应叶片的DNA进行HPT基因的PCR扩增,两者符合率达到97.04%。结果表明,利用叶片对潮霉素的反应检测转基因水稻的方法适用于不同世代、不同生育期和不同部位的叶片,具有很高的可行性和可靠性。 The transgenic study of rice is generally based on Hyglomychl Phosphobamfemse (6P6) gene HPT as a selection marker, so the reliability of hygromycin resistance testing is crucial. In this study, Zhonghua 11 and Nipponbare T1, T2 and T6 transgenic rice were used as materials to study the resistance to hygromycin in leaves at different stages of seedling and meiosis, The DNA of the corresponding leaves was extracted synchronously for PCR amplification of HPT gene, the coincidence rate reached 97.04%. The results showed that the method of detecting transgenic rice with hygromycin is suitable for different generations, different growth stages and different parts of leaves, with high feasibility and reliability.