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核糖霉素与新霉素均可能含有杂质新霉胺。新霉胺既是生产过程中的副产品,又是核糖霉素与新霉素B、C的降解产物,具有一定的毒性,因此在成品中控制新霉胺含量很有必要。中国药典和英国药典制定了新霉素中新霉胺的限度检查方法,沈国孚等曾用薄层色谱法对新霉素组分分析进行了初步探讨,但还未见薄层色谱法分析核糖霉素的报道,我们采用薄层扫描法对核糖霉素和新霉素中新霉胺进行了定量。 新霉素中B、C为差向异构体,结构虽极相似,但它们的生物活性和毒性却有很大差异,C的生物活性只为B的二分之一,但耳毒性却为B的两倍。Bacsa等曾报道用薄层法分离B、C,但我们反复试验,未能重现,后经摸索选出一展开系统,较成功地分离子B、C,并用面积归一化法粗略对其定量。 实验采用岛津CS—930双波长薄层扫描仪
Both neomycin and neomycin may contain impurities neotame amine. Neotametine is not only a byproduct of the production process, but also a degradation product of ribosamycin and neomycin B, C, so it is necessary to control neomycin concentration in the finished product. Chinese Pharmacopoeia and the United Kingdom Pharmacopoeia developed a neomycin neamine test limit method, Shen Guofu and other thin-layer chromatography has been neomycin component analysis conducted a preliminary study, but no thin-layer chromatography has yet to identify ribopneumonia We reported that neamine was quantified by thin layer scanning in both ribomycin and neomycin. The neomycin B, C is epimers, although the structure is very similar, but their biological activity and toxicity are very different, C biological activity is only one half of B, but ototoxicity was B twice. Bacsa et al. Reported using thin-layer method to separate B, C, but we repeated the test, failed to reproduce, after fumble through a selected system, more successful separation of B, C, and area normalization method roughly Quantitative. Shimadzu CS-930 dual-wavelength thin layer scanner was used in the experiment