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目的 研究胰岛素对心肌细胞和心肌成纤维细胞增殖的影响 ,探讨其在心肌肥厚中的作用。方法 ①乳鼠心肌细胞和心肌成纤维细胞的培养及光镜、电镜和免疫细胞化学染色的鉴定。②两种细胞在不同浓度胰岛素作用下细胞数量、代谢活性与DNA合成 (WST 1、BrdUELISA法 )的测定及细胞周期分析 (流式细胞仪 )。结果 ①培养的心脏细胞分别为心肌细胞和成纤维细胞。②胰岛素作用下 ,心肌细胞的数量、WST 1与BrdU的OD值及S +G2 +M期细胞百分比无变化 (P >0 0 5 ) ;而心肌成纤维细胞的数量、WST 1与BrdU的OD值及S +G2 +M期细胞百分比均升高 (P <0 0 1或P <0 0 5 )。结论 胰岛素可促进培养的心肌成纤维细胞增殖 ,可能在心肌肥厚中起重要作用
Objective To study the effect of insulin on the proliferation of cardiac myocytes and cardiac fibroblasts and to explore the role of insulin in cardiac hypertrophy. Methods ① Culture of neonatal rat cardiomyocytes and cardiac fibroblasts and identification by light microscopy, electron microscopy and immunocytochemical staining. ② The cell number, metabolic activity and DNA synthesis (WST 1, BrdU ELISA) and cell cycle analysis (flow cytometry) of the two cells under different concentrations of insulin. Results ① The cultured heart cells were cardiomyocytes and fibroblasts respectively. ② The number of myocardial cells, the OD of WST 1 and BrdU and the percentage of cells in S + G2 + M phase did not change under the action of insulin (P> 0.05); while the number of myocardial fibroblasts, WST 1 and BrdU OD The percentage of cells in S + G2 + M phase increased (P <0.01 or P <0.05). Conclusion Insulin can promote the proliferation of cultured cardiac fibroblasts, which may play an important role in myocardial hypertrophy