1969年以来,田边药厂已用固定化酶柱进行工业化连续生产L-氨基酸,固定化酶是由酶离子结合到DEAE-Sephadex上制得。该方法利用L-氨基酸和未水解的酰基-D-氨基酸洗脱液浓缩后的溶解度的不同而分离。因此,假如能够利用高浓度底物,可预料有减少要蒸发的溶液体积之优点。由于氨基酰化酶是通过离子结合吸附在DEAE-Sephadex上,因此若利用高浓度底物,就会释出一些酶。为了克服上述局限,以及制备价廉的固定化氨基酰化酶,该厂研究了各种固定化氨基酰化酶的方法,并报道了它的制备性质及适用性。最近发现,将丹宁共价结合到氨 Since 1969, Tanabe Pharmaceutical Factory has carried out industrial production of L-amino acids by immobilized enzyme columns, and immobilized enzymes are produced by binding of enzyme ions to DEAE-Sephadex. This method uses the separation of the L-amino acids and the un-hydrolyzed acyl-D-amino acid eluate concentration after concentration difference. Therefore, the advantage of reducing the volume of solution to be evaporated can be expected provided that a high concentration of substrate can be utilized. Since aminoacylase is adsorbed on DEAE-Sephadex by ion binding, some enzymes will be released if high substrate concentration is used. In order to overcome the above limitations and to prepare inexpensive immobilized aminoacylase, various methods of immobilizing aminoacylase were studied at the plant and its preparation properties and applicability were reported. It has recently been discovered that tannin is covalently bound to ammonia