应用单细胞凝胶电泳研究了一定剂量的亚硒酸钠对离体和在体大鼠肝细胞 DNA损伤的作用。结果表明 :在2 .185、 4.375、 8.75 0、 17.5 0 0、 35 .0 0 0 μmol/ L 的剂量条件下 ,除 2 .185 μmol/ L 的剂量外 ,其余剂量的亚硒酸钠均可引起离体大鼠肝细胞 DNA损伤。 5、 10、 2 0 μmol/ kg的亚硒酸钠也可引起在体大鼠肝细胞 DNA损伤。亚硒酸钠引起的离体和在体大鼠肝细胞 DNA损伤均存在明显的剂量 -反应关系。研究结果提示 ,一定剂量的亚硒酸钠能引起离体和在体大鼠肝细胞 DNA损伤 Single cell gel electrophoresis was used to study the effect of certain doses of sodium selenite on DNA damage in isolated and in vivo rat hepatocytes. The results showed that under the conditions of 2.185, 4.375, 8.75 0, 17.5 0 0 and 35.0000 μmol / L, except for the dose of 2.185 μmol / L, all the other doses of sodium selenite could be Lead to isolated rat liver cell DNA damage. Sodium selenite at 5, 10, 20 μmol / kg also caused DNA damage in rat hepatocytes in vivo. Sodium selenite induced in vitro and in vivo rat hepatocyte DNA damage there is a significant dose-response relationship. The results suggest that a dose of sodium selenite can cause DNA damage in isolated and in vivo rat hepatocytes