The evolution of Ancient Silk Road (ASR) was deeply influenced by late Holocene moisture changes in Arid Central Asia (ACA). Nevertheless, controversies in Holocene moisture change patt of ACA and poorly–constrained age models of related studies have made the discussion about late Holocene moisture changes in ACA and their influences on the evolution of ASR difficult. Recently, a high–resolution age model during the late Holocene was established for Kalakuli Lake, a small glacier lake located in the core area of ACA. A thorough rock magnetic investigation was carried out on Kalakuli Lake sediments based on this age model. The magnetic mineral assemblage of Kalakuli Lake sediments is still dominated by primary magnetite despite minor diagenetic effects. Comparisons of rock magnetic records to parameters previously used as indicator of glacier fluctuations suggest that clastic input to Kalakuli Lake was high (low) and magnetic grain size is relatively larger (smaller), when glaciers on Muztagh Ata advanced (retreated). The ARM/SIRM ratio, a magnetic grain size proxy, is directly related to lake hydrodynamics, which are ultimately controlled by glacier fluctuations on Muztagh Ata as the result of regional moisture changes. Late Holocene moisture changes indicated by the ARM/SIRM ratio are consistent with cool/wet and warm/dry oscillations indicated by the unweighted average of biomarker hydrogen isotopic data of the C26 and C28 n–alkanoic acids in a previous study about Kalakuli Lake, most moisture change records of the core area of ACA and winter insolation of the North Hemisphere, but opposite to Asian monsoon evolution. Given Asian monsoon and the westerlies are mutually inhibited, we propose that late Holocene moisture changes in the core area of ACA were controlled by the intensity of Asian monsoon versus the westerlies under the govance of solar insolation. Generally increased moisture since the late Holocene indicated by the ARM/SIRM ratio favored cultural exchange and integration between the west and the east Eurasia, which paved the way for the formation of ASR. Coincidence between significant increase in moisture at ～200 BC suggested by the ARM/SIRM ratio and the formation of ASR indicates moisture as an important factor that facilitated the formation of ASR. The onsets of three prosperity periods of ASR in the history generally correspond to periods when moisture was relatively high, nevertheless, stagnant periods of ASR do not coincide with periods when moisture was relatively low in the core area of ACA. Disorganized correlations between stagnant periods of ASR and moisture changes in the core area of ACA suggest that moisture is not the decisive factor influencing the evolution of ASR.