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体外培养的大鼠胚胎中脑腹侧(VM)细胞和大脑皮质神经干细胞(NSCs)分别移植入帕金森病(PD)模型大鼠毁损侧纹状体。移植后2,4,8周采用脑微透析术结合高效液相色谱(HPLC)动态监测毁损侧纹状体内多巴胺水平,同期观察大鼠的旋转行为。移植后8周采用免疫组化法检测植入细胞的存活及分化情况。结果显示:移植后4,8周VM移植组多巴胺水平明显高于NSCs移植组或对照组,同期VM移植组较其余两组旋转行为有显著改善,而NSCs移植组与对照组多巴胺水平及旋转行为无显著差异。VM移植组较NSCs移植组植入的细胞易存活和分化。以上结果提示,大鼠胚胎VM细胞移植治疗PD模型的疗效明显优于胚胎大脑皮质NSCs。
In vitro cultured rat embryo midbrain ventral (VM) cells and cerebral cortex neural stem cells (NSCs) were implanted into Parkinson’s disease (PD) model rats to destroy the lateral striatum. At 2, 4, and 8 weeks after transplantation, dopamine levels in the striatum were dynamically monitored by brain microdialysis coupled with high performance liquid chromatography (HPLC), and the rotation behavior of rats was observed at the same time. Eight weeks after transplantation, the survival and differentiation of the implanted cells were examined by immunohistochemistry. The results showed that the levels of dopamine in VM group at 4 and 8 weeks after transplantation were significantly higher than those in NSCs transplantation group or control group. In the same period, the rotation behavior of VM group was significantly improved compared with the other two groups. However, the dopamine level and rotational behavior of NSCs transplantation group and control group No significant difference. VM transplantation group than NSCs transplantation group of cells easy to survive and differentiation. These results suggest that rat embryonic VM cell transplantation in the treatment of PD model was significantly better than the embryonic cerebral cortex NSCs.