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丙型肝炎病毒(HCV)是一种单股正链RNA病毒,是导致慢性肝炎的主要病原体之一.近年体外培养体系成功构建后HCV分子生物学的研究获得重要进展,HCV生活周期的各个步骤得到了深入研究.由于NS2蛋白既不是病毒粒子结构成分又不是病毒RNA复制必需成分,因此,近年有较多研究关注于NS2对病毒包装的影响.突变分析表明,NS2蛋白的N端保守位点及跨膜结构对病毒包装至关重要,C端蛋白酶蛋白酶结构域而非其蛋白酶活性为病毒包装所需.此外,NS2蛋白与病毒结构蛋白,非结构蛋白以及多种宿主因子存在相互作用,并在病毒包装过程中形成多种复合体,本文对这些研究进行了归纳总结.
Hepatitis C virus (HCV) is a single-stranded positive-stranded RNA virus and is one of the major pathogens causing chronic hepatitis C. In recent years, significant progress has been made in the molecular biology of HCV after successful construction of the in vitro culture system. The steps of HCV life cycle In recent years, more and more attention has been paid to the effect of NS2 on viral packaging.Mutation analysis showed that the N-terminal conserved site of NS2 protein And the transmembrane structure are essential for viral packaging and the C-terminal protease domain rather than its protease activity is required for viral packaging.In addition, the NS2 protein interacts with viral structural proteins, non-structural proteins, and various host factors and In the virus packaging process to form a variety of complex, the paper summarizes these studies.