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目的 为探索精子作为外源基因的载体建立转基因小鼠的可行性。 方法 我们从性成熟小鼠的输精管及部分附睾管中取出精子,与阳离子脂质体包裹的DNA混合孵育之后将其注入超排卵小鼠的阴道内,48 h后收集II细胞期胚胎细胞。结果 (1) 精子可转染上外源DNA,转染率达53%;(2) 转染有外源DNA的精子可使II细胞期胚胎携带上外源基因,携带率为11.5%。结论 小鼠输精管及附睾管内的精子可被脂质体包裹的外源DNA转染并可作为载体将此外源DNA带入早期胚胎。
Objective To explore the feasibility of establishing transgenic mice by using sperm as a foreign gene. METHODS: Sperm were removed from the vas deferens and some of the epididymal ducts of sexually mature mice. After incubation with mixed liposome-encapsulated DNA, they were injected into the vagina of superovulated mice and harvested for stage II cells after 48 h. Results (1) Sperm transfected with exogenous DNA, the transfection rate was 53%. (2) Exogenous DNA transfected spermatozoa could carry exogenous genes in II cell stage embryos with a carriage rate of 11.5%. Conclusion Sperm in mouse vas deferens and epididymal tubes can be transfected with liposome-encapsulated exogenous DNA and can be used as a vector to transfer the exogenous DNA into early embryos.