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目的:研究人工模拟干旱胁迫条件下甘草种源种子萌发特性的地理变异规律,探讨地理变异模式和生态学机制,为甘草种子区划和种子调拨奠定理论基础。方法:现地调查收集种源种子材料,采用PEG法设置水势梯度,通过室内发芽实验观察种子萌发特性。结果:各种源种子的发芽能力对水势降低的敏感程度存在相当显著的差异,总体呈现随着地理经度的增加,种子发芽对干旱胁迫的敏感性逐渐增加的变异趋势。与气候因子的相关分析结果表明,年均温低,年降水充沛地区种源的种子发芽对干旱胁迫敏感,而年降水量少、年均温高的地区的种源种子发芽对干旱胁迫的不敏感。结论:干旱胁迫条件下甘草种源种子萌发特性存在显著的地理变异,地理变异的形成是自然选择作用的结果。
OBJECTIVE: To study the geographical variation of germination characteristics of Glycyrrhiza uralensis seed germination under artificial simulated drought stress, and to explore the geographical variation pattern and ecological mechanism to lay a theoretical foundation for licorice seed division and seed allocation. Methods: The seed germplasm materials were collected and investigated. The water potential gradient was set by PEG method. The germination characteristics of seeds were observed by indoor germination experiment. Results: There was a significant difference in the sensitivity of seed germination ability to water potential reduction. Overall, the sensitivity of seed germination to drought stress gradually increased with the increase of geographical longitude. The results of correlation analysis with climatic factors showed that seed germination of seed germination in areas with low annual average temperature and abundant annual rainfall was sensitive to drought stress, while seed germination in areas with less annual precipitation and annual mean temperature was less sensitive to drought stress sensitive. Conclusion: The germination characteristics of Glycyrrhiza uralensis seed germplasm under drought stress have significant geographical variation, and the formation of geographical variation is the result of natural selection.