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为了将光遗传技术用于自由移动小动物的在体神经调控,本文研制了具有多通道输出、微型化、无线程控参数可调的光刺激器.采用μLED作为多通道输出光源,使用无线集成芯片实现恒流驱动和亮度连续可调,以射频通讯方式实现刺激参数程控,实时调节光刺激频率、幅度和占空比.采用双层电路板和贴片工艺来减小尺寸和重量.光刺激器体积为20 mm×16 mm×5 mm,重量(包括电池)3.3 g,通信距离10 m.实验结果显示该光刺激器输出光功率强度足够激活核团内光敏神经元,在转基因小鼠次级运动皮层脑区施加频率为30 Hz光刺激,可观察到小鼠运动改变.研制的刺激器可推动光遗传技术在自由移动小鼠脑深部和脊髓在体神经调控领域的应用.
In order to use optical genetic technology for the regulation of free-moving small animals in vivo, this paper developed a light stimulator with multi-channel output, miniaturization and wireless programmable parameters.Using μLED as multi-channel output light source, using wireless integrated chip Constant current drive and brightness are continuously adjustable, and the stimulus parameters are programmed by radio frequency communication to adjust the frequency, amplitude and duty ratio of the light stimulus in real time. The double circuit board and the chip process are adopted to reduce the size and the weight. The volume is 20 mm × 16 mm × 5 mm, the weight (including the battery) is 3.3 g, the communication distance is 10 m.The experimental results show that the light output intensity of the light stimulator is enough to activate photosensitive neurons in the nucleus, The motor cortical area was exposed to light at a frequency of 30 Hz and the change of mouse motion was observed.The stimulator developed could promote the application of optogenetics in the deep brain of free-moving mice and the spinal nerves in vivo.