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目的了解梅州市小儿腹泻中病毒性感染的病原。方法2008年4月~2009年3月先后到梅州市各医院收集568例腹泻患儿粪便标本进行检测,轮状病毒用酶免疫试验,杯状病毒和星状病毒用逆转录-聚合酶链反应法进行检测。结果在568份标本中,轮状病毒(RV)阳性238份,阳性率为41.9%。7月~1岁年龄段的患儿星状病毒阳性率较高;12月~1月份星状病毒阳性率较高。结论在梅州地区病毒性腹泻的病原是复杂的,轮状病毒的感染最为严重,其中杯状病毒与星状病毒的病例也占用很重要的地位。
Objective To understand the pathogen of viral infection in infantile diarrhea in Meizhou. Methods From April 2008 to March 2009, 568 cases of diarrhea in children were collected from all the hospitals in Meizhou City for detection. Rotavirus was tested by enzyme immunoassay. Calicivirus and astrovirus were detected by reverse transcription - polymerase chain reaction Law for testing. Results In 568 specimens, rotavirus (RV) positive 238, the positive rate was 41.9%. The positive rate of astrovirus in children aged from July to 1 was high, and the positive rate of astrovirus was higher from December to January. Conclusions The pathogen of viral diarrhea in Meizhou is complex and the rotavirus is the most serious one. The cases of calicivirus and astrovirus also occupy a very important position.