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目的:建立养血生发胶囊240 nm和310 nm的双波长融合HPLC特征图谱。方法:样品色谱测定使用Agilent ZORBAX Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),柱温30℃,以0.1%冰醋酸-乙腈为流动相进行梯度洗脱,同时采集2个特征吸收波长下的信号,记录10批样品的HPLC特征图谱;采用“中药色谱指纹图谱超信息特征数字化评价系统”对图谱进行融合分析。结果:以大黄素为参照物,10批样品所得融合特征图谱共标定18个共有峰,相似度均在0.93以上。方法的精密度、稳定性和重复性良好。结论:本试验建立的方法克服了单波长检测时信息量有限的缺点,能较全面、真实地揭示中药材的内在质量特征,可为养血生发胶囊的质量控制和评价提供依据。
OBJECTIVE: To establish a dual-wavelength fusion HPLC profile of Nanshenshangfa capsule at 240 nm and 310 nm. Methods: The chromatographic determination of samples was performed on a Agilent ZORBAX Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) with a column temperature of 30 ° C and gradient elution with 0.1% glacial acetic acid-acetonitrile as the mobile phase. Two characteristic absorptions Wavelength of the signal, record 10 batches of samples of HPLC characteristics of the map; using “Chinese medicine chromatographic fingerprint characteristics of ultra-information digital evaluation system,” the fusion analysis of the map. Results: Emodin was used as a reference and the eigenvalues obtained from 10 batches of samples were scaled to 18 common peaks with similarities of 0.93. The method has good precision, stability and repeatability. Conclusion: The method established in this study overcomes the shortcomings of limited information in single wavelength detection and reveals the intrinsic quality characteristics of traditional Chinese medicine more comprehensively and truthfully, which may provide the basis for the quality control and evaluation of nourishing capsule.