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目的探讨结核杆菌纯化蛋白衍生物（ＰＰＤ）在人脐静脉内皮细胞（ＥＣ）对外周血单个核细胞（ＰＭＮＣ ）粘附过程中的作用。方法利用培养的人脐静脉内皮细胞株，测定人ＰＭＮＣ和ＥＣ的粘附率；采用ＭｃＡｂＣＤ６２Ｅ抑制试验，检测ＰＰＤ对ＥＣ表达粘附分子的作用。结果经ＰＰＤ处理的 ＥＣ对ＰＭＮＣ的粘附率增加，并有一定的时相关系； ＭｃＡｂＣＤ６２Ｅ可以降低 ＰＰＤ的ＥＣ和ＰＭＮＣ的粘附率。结论ＰＰＤ可以直接刺激ＥＣ表达粘附分子，进一步参与介导白细胞与内皮细胞的粘附。
Objective To investigate the role of Mycobacterium tuberculosis purified protein derivative (PPD) in the adhesion of human umbilical vein endothelial cells (ECs) to peripheral blood mononuclear cells (PMNC). Methods The cultured human umbilical vein endothelial cell line was used to determine the adherence rate of human PMNC and EC. The effect of PPD on EC expression of adhesion molecules was tested by McAbCD62E inhibition. Results The adhesion rate of PMNC treated with PPD increased and there was a certain time-dependent relationship. McAbCD62E could reduce the adhesion rate of PPD EC and PMNC. Conclusion PPD can directly stimulate EC expression of adhesion molecules, and further participate in the mediation of leukocyte and endothelial cell adhesion.