论文部分内容阅读
旨在阐明胁迫条件对一土生青霉菌总酚积累及其抗氧化活性的影响。采用固体培养基培养青霉菌,以紫外线辐射、添加H2O2水溶液和降低培养基营养物质含量作为胁迫手段,检测受到胁迫后,菌体总酚的积累及酚类清除自由基的能力。菌丝体总酚含量以Folin-Ciocalteu法测定,自由基清除率以分光光度法测定。结果表明,在胁迫条件下,各实验组菌丝体的总酚含量较对照组均有显著提高,添加H2O2组的总酚含量最高达63.86mg/g,紫外线辐射组的总酚含量最高达58.4mg/g,营养胁迫组的总酚含量最高达43.19mg/g。各实验组酚类提取物对羟自由基的清除率最高,其中添加1mmol/LH2O2组为35.28%,紫外线辐射40s组为69.97%,75%营养胁迫组为50.83%。因此,胁迫条件可增加该青霉合成酚类化合物及提高其抵抗胁迫的能力。
The aim was to elucidate the effect of stress conditions on the accumulation of total phenols and their antioxidant activity in Penicillium decumbensi. Penicillium sp. Was cultivated in solid medium. The UV-radiation, aqueous H2O2 solution and nutrient content of medium were used as stress measures to detect the accumulation of total phenols and phenolic scavenging free radicals after stress. The mycelial total phenolic content was measured by the Folin-Ciocalteu method and the free radical scavenging rate was determined spectrophotometrically. The results showed that under stress conditions, the total phenols content in mycelium of experimental groups was significantly higher than that of control group. The total phenolic content of H2O2 group was up to 63.86 mg / g, and the total phenols content of UV radiation group was up to 58.4 mg / g, the total phenolic content of the nutrient stress group reached 43.19mg / g. The phenolic extracts of each experimental group had the highest rate of hydroxyl radical scavenging, which was 35.28% in 1mmol / LH2O2 group, 69.97% in 40s UV irradiation group and 50.83% in 75% nutrition group. Therefore, stress conditions can increase the Penicillium synthesis of phenolic compounds and increase their ability to resist stress.