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目的:了解小儿急性呼吸道感染与人博卡病毒(Human Bocavirus,HBoV)的相关性。方法:对2008年1～5月在长春市儿童医院住院的1007例急性呼吸道感染患儿鼻咽深部分泌物样本用PCR扩增的方法进行HboV NS1基因检测。随机选取8例HBoV阳性产物进行测序,并将测到的序列与GenBank中的基因序列和HBoV阳性病例的临床资料进行分析。结果:1007例标本共检测出44例HBoV阳性,阳性率为4.37%,其中急性上呼吸道感染、急性支气管炎、毛细支气管炎和支气管肺炎患儿人博卡病毒阳性检出率分别为1.29%(2/155)、5.71%(2/35)、8.70%(2/23)和4.79%(38/794)。HboV急性呼吸道感染的年龄分布为1岁以下39例,占88.64%;1岁以上5例,占11.36%;男女性别比值是1.75∶1;临床表现咳嗽伴喘憋占97.56%,咳嗽伴腹泻占34.14%,入院时体温升高占54.55%,肺部听诊闻及干和/或湿性罗音者占95.12%,X线胸片片状影占87.80%,病程平均为13.79天。结论:人博卡病毒是引起小儿急性呼吸道感染的病原之一;HboV所致呼吸道感染部位多在下呼吸道;感染患儿以1岁以内婴儿为主。
Objective: To understand the correlation between acute respiratory infection in children and human Bocavirus (HBoV). Methods: HboV NS1 gene was detected by PCR amplification in deep nasopharyngeal secretions of 1007 children with acute respiratory infection hospitalized in Changchun Children’s Hospital from January to May in 2008. Eight HBoV positive products were randomly selected and sequenced. The sequences were analyzed with the gene sequences in GenBank and the clinical data of HBoV positive cases. Results: A total of 44 cases of HBoV positive were detected in 1007 cases, the positive rate was 4.37%. The positive rates of Boca virus in children with acute upper respiratory tract infection, acute bronchitis, bronchiolitis and bronchopneumonia were 1.29% 2/155), 5.71% (2/35), 8.70% (2/23) and 4.79% (38/794). The age distribution of HboV Acute respiratory tract infection was 39 cases under 1 year old, accounting for 88.64%; 5 cases over 1 year old, accounting for 11.36%; the ratio of male to female was 1.75:1; clinical manifestations cough with wheezing accounted for 97.56%, cough with diarrhea accounted 34.14%. The body temperature increased 54.55% on admission, 95.12% of the patients heard auscultation, dry and / or wet rales, and 87.80% of X-ray films showed an average duration of 13.79 days. Conclusion: Bocavirus is one of the pathogens causing acute respiratory infection in children. Most of the respiratory tract infections caused by HboV are in the lower respiratory tract. Infants with infection are mainly infants under 1 year old.