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背景:运动性疲劳作为一种应激源,必然会引起脑内某些神经核团物质和功能的变化,哪些核团与运动性疲劳关系密切,哪些物质介导了疲劳的中枢神经功能和/或结构的改变,目前尚不清楚。目的:研究下丘脑腹内侧核和背内侧核神经元中一氧化氮合酶与运动性疲劳的关系。设计:随机对照实验。材料:实验于2003-10/2004-01在中北大学和山西医科大学人体解剖学教研室完成。选择雄性W ister大鼠20只,清洁级。干预:动物随机分为实验组10只,对照组10只。实验组每天经大运动量游泳达到力竭状态,并连续4周,制成运动性疲劳动物模型。造模完成后用ABC 免疫组织化学方法观测下丘脑腹内侧核和背内侧核神经元中神经元型一氧化氮合酶表达状况,并进行图像分析和统计学处理。主要观察指标:单位视野中神经元型一氧化氮合酶阳性细胞个数、阳性物面积和灰度。结果:疲劳组下丘脑腹腔内侧核中神经元型一氧化氮合酶阳性细胞数量为(25.25±7.35)个/视野,显著大于对照组(9.70±3.20)个/视野(P <0.001);神经元型一氧化氮合酶阳性物面积为(3867.75±1940.41)μm2/视野,显著大于对照组(750.13±579.88)μm 2/视野(P<0.001);疲劳组背内侧核中神经元型一氧化氮合酶阳性细胞数量为(30.25±7.87)个/视野,显著大于对照组(14.00±4.99)个/视野(P <0.001);神经元型一氧化氮合酶阳性物面积为(4512.06±1243.93) μm 2/视野,显著大于对照组(782.46±711.46)μm 2/视野(P <0.001)。结论:下丘脑腹内侧核和背内侧核神经元中的神经元型一氧化氮合酶阳性神经元与中枢运动性疲劳的形成密切相关,一氧化氮可能在下丘脑腹内侧核和背内侧核对疲劳应激反应的调节中发挥重要作用。
BACKGROUND: As a kind of stressor, exercise-induced fatigue inevitably leads to the changes of substances and functions of some nerve nuclei in the brain, which nuclei are closely related to exercise-induced fatigue and which substances mediate the fatigue of central nervous system function and / Or structural changes, it is not clear. Objective: To investigate the relationship between nitric oxide synthase (NOS) and exercise-induced fatigue in hypothalamic ventromedial and dorsal medulla neurons. Design: Randomized controlled experiment. MATERIALS: The experiment was performed at Department of Human Anatomy, North China University and Shanxi Medical University from October 2003 to January 2004. Twenty male Wistar rats were selected and cleaned. Intervention: The animals were randomly divided into experimental group 10, control group 10. The experimental group reached the state of exhaustion by swimming a large amount of exercise every day for 4 weeks and made a model of exercise-induced fatigue. After modeling, the expression of neuronal nitric oxide synthase in hypothalamic ventromedial and dorsal medullary nucleus neurons was observed by ABC immunohistochemical method, and image analysis and statistical analysis were performed. MAIN OUTCOME MEASURES: Number of neuronal nitric oxide synthase positive cells per unit area, area of positives and gray scale. Results: The number of neuronal nitric oxide synthase positive cells in hypothalamus medial nucleus in fatigue group was (25.25 ± 7.35) / visual field, which was significantly higher than that in control group (9.70 ± 3.20) / visual field (P <0.001) (3867.75 ± 1940.41) μm 2 / field of view, which was significantly higher than that of the control group (750.13 ± 579.88) μm 2 / field of vision (P <0.001). The neuronal nitric oxide The number of positive cells of nitric oxide synthase was (30.25 ± 7.87) / visual field, which was significantly higher than that of the control group (14.00 ± 4.99) / visual field (P <0.001). The positive area of neuronal nitric oxide synthase was (4512.06 ± 1243.93) ) μm 2 / field of view, significantly greater than the control group (782.46 ± 711.46) μm 2 / field of vision (P <0.001). CONCLUSIONS: Neuronal nitric oxide synthase (NOS) -positive neurons in the hypothalamic ventromedial and dorsal medullary nuclei are closely related to the formation of central motor fatigue. Nitric oxide may be responsible for fatigue in the hypothalamic ventromedial and dorsal medial nuclei Stress regulation plays an important role in regulation.