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菠菜放氧的ＰＳＩ核心复合物经０．８ｍｏｌ／ＬＴｒｉｓ－ＨＣｌ（ｐＨ８．０）洗涤后，用温和的非离子去垢剂ＤＭ和高浓度的ＬｉＣｌＯ４增溶，再经ＤＥＡＥ－Ｔｏｙｏｐｅａｒｌ－６５０Ｓ离子交换柱层析分离，可得到ＰＳＩＩ核心天线４３ｋＤ叶绿素ａ结合蛋白（ＣＰ４３）。ＳＤＳ－ＰＡＧＥ显示一条４３ｋＤ蛋白质带。根据Ａｒｎｏｎ法和Ｍａｒｋｗｅｌ法的结果表明，每个蛋白质分子结合有２０～２１个分子的叶绿素ａ。室温条件下，ＣＰ４３在红光区具有６７１ｎｍ的最大吸收峰和６８３ｎｍ的荧光发射峰，以及Ｗ型的圆二色信号，表明其处于较为天然的状态。文中还制备并鉴定了对ＣＰ４３特异的抗血清。
The spinach oxygen-releasing PSI core complexes were washed with 0.8 mol / L Tris-HCl (pH 8.0), solubilized with mild non-ionic detergent DM and high concentration of LiClO 4, and then subjected to DEAE-Toyopearl-650S ion exchange Column chromatographic separation, PSII core antenna 43kD chlorophyll a binding protein (CP43). SDS-PAGE shows a 43 kD protein band. According to the results of Arnon and Markwel methods, there are 20 to 21 molecules of chlorophyll a bound to each protein molecule. At room temperature, CP43 has a maximum 671 nm peak and a 683 nm peak in the red region, and a circular dichroic signal of type W, indicating that it is in a more natural state. CP43 specific antisera were also prepared and identified.