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目的研究某污灌区玉米中有机污染物对小鼠肝肾组织的氧化损伤作用。方法采用超声振荡法提取某污灌区农田生产的玉米中的有机污染物,对小鼠进行灌胃染毒2周;测定肝、肾组织的总超氧化物歧化酶(T-SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)。结果污灌区低、高剂量组肝组织的T-SOD活性分别为(32.44±7.93),(30.92±9.29)U/(mg.prot);肾组织的GSH-PX活性分别为(67.28±27.21),(66.52±15.19)U/(mg.prot),与对照组比较均明显降低(P<0.05或P<0.01)。结论该污灌区玉米有机提取物中含有致小鼠肝肾组织氧化损伤的有机污染物;对照区玉米中的有机污染物很可能来源于被污染的大气。
Objective To study the oxidative damage of liver and kidney in mice induced by organic pollutants in a sewage irrigation area. Methods The organic pollutants in maize produced in farmland of a sewage irrigation area were extracted by ultrasonic vibration method and the mice were gavaged for 2 weeks. The contents of total superoxide dismutase (T-SOD) and malondialdehyde Aldehyde (MDA), glutathione peroxidase (GSH-Px). Results The T-SOD activities in liver tissue of low and high dose groups were (32.44 ± 7.93) and (30.92 ± 9.29) U / (mg.prot), respectively. The GSH-PX activities in renal tissue were (67.28 ± 27.21) , (66.52 ± 15.19) U / (mg.prot) respectively, which were significantly lower than those in the control group (P <0.05 or P <0.01). Conclusion The organic extracts of maize in the sewage irrigation area contain organic pollutants that cause oxidative damage in mouse liver and kidney tissues. The organic pollutants in maize in the control area are likely to originate from the polluted atmosphere.