目的:研究自拟醒脑除颤方对6-羟基多巴诱导帕金森病模型大鼠的增效减毒作用。方法:将SD大鼠的大脑定位于内侧前脑束(Medial forebrain bundle,MFB)部位再注入6-羟基多巴(24μg)制成PD模型大鼠。再把成功的PD大鼠随机分为5组,每组10只,雌雄各半,即模型组、美多巴组(75 mg/kg)、左旋多巴组(60 mg/kg)、β-细辛醚组(15 mg/kg)、自拟醒脑除颤方组(左旋多巴60 mg/kg+β-细辛醚15 mg/kg)。给药组按上述剂量灌胃给药,1 d 2次,给药30 d。给药结束观察各组5 min内的AIM评分(口面部、轴性和前肢部位),计算肝肾系数和HE染色观察纹状体的组织病理形态。结果:与假手术组比较,模型组的口面部、轴性和前肢的AIM评分都显著增加(P<0.01),肝肾系数无显著差异(P>0.05),纹状体中神经细胞较少,残留神经元核固缩,部分细胞胞浆明显肿胀,这说明了PD大鼠造模成功和造模剂6-羟基多巴对模型大鼠无肝肾毒性。与模型组比较,各给药组的AIM评分都显著减少(P<0.01),给药治疗后纹状体神经细胞数略有增多,结构基本正常,无明显炎性浸润变性及坏死现象,其中左旋多巴组的肾脏系数显著增加(P<0.05)。与左旋多巴组比较,美多巴组和自拟醒脑除颤方组的口面部、轴性和前肢的AIM评分及右肾系数都显著减少(P<0.05)。结论:自拟醒脑除颤方对6-羟基多巴诱导帕金森病模型大鼠具有一定增效减毒的作用。 OBJECTIVE: To study the synergistic and attenuated effects of self-designed defibrillation and defibrillators on 6-hydroxydopamine-induced Parkinson’s disease model rats. Methods: The brain of SD rats was placed in the medial forebrain bundle (MFB) and then injected with 6-hydroxydopamine (24μg) into PD model rats. The successful PD rats were randomly divided into 5 groups, 10 in each group, male and female in half, namely model group, mededopa group (75 mg / kg), levodopa group (60 mg / kg) Asarone group (15 mg / kg), self-conscious group of defibrillation (levodopa 60 mg / kg + β-asarone 15 mg / kg). Administration group according to the above dose gavage, 1 d 2 times, administration 30 d. At the end of the treatment, the AIM scores (facial, axial and forelimb parts) within 5 min of each group were observed. The hepatic and renal factors were calculated and the histopathology of the striatum was observed by HE staining. Results: Compared with the sham operation group, the AIM scores of oral, axial and forelimb of the model group were significantly increased (P <0.01), but there was no significant difference between the liver and kidney (P> 0.05) , Nuclear remnants of residual neurons and some cytoplasm were obviously swollen, indicating that PD model rats succeeded in modeling and that model drug 6-hydroxydopamine did not have hepatotoxicity to model rats. Compared with the model group, the AIM score of each administration group was significantly decreased (P <0.01). After administration, the number of neurons in the striatum increased slightly, the structure was basically normal, and there was no obvious inflammatory infiltration degeneration and necrosis Renal coefficient was significantly increased in levodopa group (P <0.05). Compared with levodopa group, the AIM score and right kidney coefficient of oral, axial and forelimb groups in the two groups were significantly decreased (P <0.05). CONCLUSION: The self-designed defibrillation and defibrillation therapy can attenuate the effects of 6-OHDA on rats with Parkinson’s disease.