论文部分内容阅读
目的:观察不同证候H22荷瘤小鼠肿瘤组织ABC转运蛋白家族基因表达的特征。方法:对来源于早期邪毒壅盛和单纯气虚、中期阳气虚和中晚期气阴阳虚四种证候小鼠H22肿瘤组织4张Affymetrix GeneChip Mouse Exon 1.0 STArray数据标准化处理后,筛选其中的ABC转运蛋白基因进行差异分析。结果:从芯片中共筛选出与ABC转运蛋白有关的基因47个,以邪毒壅盛小鼠肿瘤基因转录最活跃,气虚次之,阳气虚最低;四种常见证候间共有25个基因转录具有明显差异;Abce1和Abcf1等7个基因中在四种常见证候肿瘤中转录水平最高。结论:Tap1(Abcb2)和Abcd2等6个高表达基因可能是邪毒壅盛型小鼠肿瘤增长快的重要因素;不同证候荷瘤小鼠肿瘤组织中的ABC转运蛋白基因转录既存在一定共性又存在很大差异,进一步证明了H22荷瘤小鼠证候客观存在的物质基础,为中医辨证论治提供分子依据。
Objective: To observe the expression of ABC transporter family gene in tumor tissues of H22 tumor-bearing mice with different syndromes. METHODS: Four Affymetrix GeneChip Mouse Exon 1.0 STArray data from four kinds of syndrome H22 tumor tissues, including early cult poison venom Qi Sheng, simple qi deficiency syndrome, mid-term yang deficiency syndrome and mid-late qi yang deficiency syndrome were normalized and screened for ABC transport Protein gene for differential analysis. RESULTS: Forty-seven genes related to ABC transporter were screened out from the chip, and the most active gene transcription was found in the mice infected with the venom and the lowest qi deficiency. The 25 common genes among the four common syndromes had transcriptional Significant differences; Abce1 and Abcf1 other seven genes in the four common syndromes in the highest level of transcription. CONCLUSION: Six highly expressed genes, Tap1 (Abcb2) and Abcd2, may play an important role in the rapid tumor growth in mice infected with Epstein-Barr virus. There are some common features of ABC transporter gene transcription in tumor tissues of different syndromes And there is a great difference, further evidence of H22 tumor-bearing mice objective existence of the material basis for the syndrome differentiation of TCM provide a molecular basis.