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本研究以光化学固定的方法,将TNF-α/IFN-γ共同偶联到24孔聚苯乙烯基板上,利用JC-1为荧光探针,采用流式细胞术在细胞水平检测共固定化及游离细胞因子诱导人宫颈癌HeLa细胞凋亡中线粒体膜电位(△Ψm)变化,进一步探讨共固定化细胞因子与游离细胞因子诱导人宫颈癌HeLa细胞凋亡的作用机制。实验结果显示,共固定化细胞因子诱导HeLa细胞凋亡不存在剂量效应关系,低剂量(20ng/ml)光固定化细胞因子在6d内能使HeLa细胞线粒体膜电位持续下降,具有长效活性,并表明该凋亡过程与线粒体途径密切相关。
In this study, photochemically immobilized TNF-α / IFN-γ was co-coupled to a 24-well polystyrene substrate. JC-1 was used as a fluorescent probe and flow cytometry was used to detect co-immobilization at the cellular level. (△ Ψm) in apoptosis of human cervical cancer HeLa cells induced by free cytokines, and further explore the mechanism of apoptosis induced by co-immobilized cytokines and free cytokines in human cervical cancer HeLa cells. The experimental results showed that there was no dose-effect relationship between HeLa cells and co-immobilized cytokines. The mitochondrial membrane potential of HeLa cells decreased continuously with low dose (20ng / ml) of light-fixed cytokines within 6 days, The results indicated that the apoptotic process was closely related to the mitochondrial pathway.