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目的应用基于ITO叉指电极微流芯片的电化学测菌法,探讨该方法检测变形链球菌的可行性。方法利用基于ITO叉指电极微流芯片的电化学测菌法检测变形链球菌,建立特定频率下细菌浓度与系统电阻抗值之间的函数关系,依据上述函数关系和测得的电化学阻抗值,分析待检样品内变形链球菌的浓度。结果在1.2KHz固定频率下、变形链球菌浓度为104～109cells/ml时,所测细菌浓度的对数与系统电阻抗值呈良好的线性关系(RITO2=0.995);从进样到获得稳定阻抗数值的时间为6分钟;进样速度采用1ml/h时电化学测菌法的灵敏度最高。结论基于ITO叉指电极微流芯片的电化学测菌法,可为变形链球菌的快速检测提供新的途径。
OBJECTIVE To evaluate the feasibility of this method for the detection of Streptococcus mutans by electrochemical detection of microfluidic chips based on ITO interdigital electrodes. Methods Mutans streptococci were detected by electrochemical detection of microfluidic chip based on ITO interdigitated electrodes. The relationship between bacterial concentration and system electrical impedance at a specific frequency was established. Based on the above functional relationship and the measured electrochemical impedance , Analyze the concentration of Streptococcus mutans in the sample to be tested. Results When the concentration of Streptococcus mutans was 104 ~ 109cells / ml at a fixed frequency of 1.2KHz, the logarithm of the measured bacterial concentration showed a good linear relationship with the system electrical impedance (RITO2 = 0.995). From the injection to obtain a stable impedance The value of the time for 6 minutes; injection speed of 1ml / h electrochemistry bacteria highest sensitivity. Conclusion The electrochemical detection of microfluidic chip based on ITO interdigital electrode can provide a new approach for the rapid detection of Streptococcus mutans.