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目的:克隆Ss-A／Ro 60 ku亚单位编码基因,检测Ss-A／Ro 60 ku亚单位及其变异体在耐药胃癌细胞中的表达情况．方法:应用RT-PCR克隆Ss-A／Ro 60 ku亚单位编码基因,应用半定量RT-PCR检测Ss-A／Ro 60 ku亚单位及其变异体在胃癌细胞中的表达情况．结果:成功克隆了Ss-A／Ro 60 ku亚单位编码基因,并发现了他的两种变异体,长度分别为52 bp和41 bp．半定量RT- PCR结果显示,Ss-A／Ro 60 ku亚单位在SGC7901／VCR中的表达强度高于SGC7901细胞(P=0．0001),Ss-A／Ro 60 ku亚单位的两种变异体在SGC7901／VCR中的表达强度也高于 SGC7901细胞(P=0．001)．结论:Ss-A／Ro 60 ku亚单位及其两种变异体为胃癌多药耐药相关分子．
OBJECTIVE: To clone the gene encoding Ss-A / Ro 60 ku subunit and detect the expression of Ss-A / Ro 60 ku subunit and its variants in drug-resistant gastric cancer cells. Methods: The Ss-A / Ro 60 ku subunit encoding gene was cloned by RT-PCR and the expression of Ss-A / Ro 60 ku subunit and its variant in gastric cancer cells was detected by semi-quantitative RT-PCR. Results: The Ss-A / Ro 60 ku subunit coding gene was successfully cloned and two variants of Ss-A / Ro 60 ku were found. The lengths of the two variants were 52 bp and 41 bp, respectively. Semi-quantitative RT-PCR results showed that the expression level of Ss-A / Ro 60 ku subunit in SGC7901 / VCR was higher than that in SGC7901 cells (P = 0.0001) and Ss-A / Ro 60 ku subunits The expression level of SGC7901 / VCR in SGC7901 cells was also higher than that in SGC7901 cells (P = 0.001). Conclusion: The Ss-A / Ro 60 ku subunit and its two variants are multidrug resistance-associated molecules in gastric cancer.