论文部分内容阅读
为检测黄瓜种传镰刀菌产生的粗毒素中镰刀菌酸含量及其对种子和幼苗的致害作用,采用乙酸乙酯和乙醚萃取法提取其中的镰刀菌酸并利用HPLC法进行定量分析,通过粗毒素处理黄瓜种子及幼苗根部测定其对种子发芽的影响和幼苗的致枯萎作用。结果表明:供试7株种传镰刀菌SC-CQ-P、HLJ-XC-S、LN-ZN203-S、GD-HY-S、HN-XH-S、LN-ZN6-S和HB-TS-R粗毒素中都检测到镰刀菌酸,质量浓度分别为3 574.71、866.43、291.10、123.32、856.13、358.27和327.71μg/mL。种传镰刀菌粗毒素对黄瓜种子发芽具有一定的抑制作用;粗毒素浸渍黄瓜幼苗根部导致幼苗枯萎,并且其对幼苗的致枯萎作用随着镰刀菌酸浓度的升高和处理时间的延长而加重。
In order to detect the content of Fusaric acid in crude toxins produced by Fusarium graminearum and its virulence to seed and seedlings, the Fusaric acid was extracted with ethyl acetate and ethyl ether and quantified by HPLC. Crude toxin treatment of cucumber seeds and seedling roots to determine the impact of seed germination and seedlings caused withered effect. The results showed that seven strains of Fusarium graminearum SC-CQ-P, HLJ-XC-S, LN-ZN203-S, GD-HY-S, HN-XH-S, LN-ZN6-S and HB-TS -R crude toxin Fusaric acid were detected, the mass concentrations were 3 574.71,866.43,291.10,123.32,856.13,358.27 and 327.71μg / mL. Inoculation with Fusarium crude toxins inhibited the germination of cucumber seedlings. The roots of cucumber seedling were immersed in the crude toxin, which caused the seedlings to wilt, and their induced withered seedling wilt increased with the increase of Fusaric acid concentration and the prolongation of treatment time .