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【目的】动物双歧杆菌RH产生的胞外多糖(Exopolysaccharides,EPS)经阴离子交换柱层析可获得EPSa和EPSb两个组分。得到可提高EPS的总产量,尤其是EPSb产量的最佳培养基和培养条件。【方法】对培养基类型、氮源、碳源、碳源浓度、培养基初始pH值、培养温度和时间对双歧杆菌EPSa和EPSb产量的影响进行分析。【结果】在初始pH值调整为7.0的含5%蔗糖的PTYG培养基上,在35°C温度下厌氧培养60 h时动物双歧杆菌RH的EPSa和EPSb产量分别为0.982±0.003 g/L和0.312±0.001 g/L。【结论】在上述条件下EPS总产量高且可获得较多的EPSb。
【OBJECTIVE】 Two fractions of EPSa and EPSb can be obtained by anion exchange column chromatography of exopolysaccharides (EPS) produced by Bifidobacterium animalis. The optimal medium and culture conditions were obtained to increase the total yield of EPS, especially EPSb production. 【Method】 The effects of medium type, nitrogen source, carbon source, carbon source concentration, medium initial pH value, culture temperature and time on the yield of EPSa and EPSb were analyzed. 【Result】 The EPSA and EPSb yields of Bifidobacterium animalis RH were 0.982 ± 0.003 g / L on the PTYG medium containing 5% sucrose with an initial pH value of 7.0 after anaerobic culture at 35 ° C for 60 h. L and 0.312 ± 0.001 g / L. 【Conclusion】 Under the above conditions, the total yield of EPS is high and more EPSb can be obtained.