目的　建立动脉内皮细胞 (ECs)的体外研究模型。方法　用消化法结合机械法的方法分离胎牛主动脉 ECs,在p H7.0的 M199培养液中培养。结果　用 因子相关抗原免疫荧光法以及倒置显微镜下细胞融合后呈单层生长、鹅卵石状排列 ,证实所培养的细胞为 ECs。在无内皮细胞生长因子的条件下体外连续培养的细胞达 18代。结论　在人动脉取材极为不易的情况下 ,胎牛主动脉 ECs的培养是很好的补充 Objective To establish an in vitro model of arterial endothelial cells (ECs). Methods The bovine aorta ECs were isolated by digestion and mechanical method and cultured in M199 culture medium of p H7.0. The results of factor-related antigen immunofluorescence and inverted microscope cells fused monolayer growth, pebbles arranged to confirm the cells cultured ECs. Cells cultured in vitro for 18 generations in vitro without endothelial cell growth factor. Conclusion It is a very good supplement to the culture of fetal bovine aortic ECs that the extraction of human arteries is extremely difficult