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目的探讨壹期煤工尘肺(CWP)患者血清氧化应激指标血红素氧合酶-1(HO-1)、核因子E2相关因子2(Nrf2)、总抗氧化能力(T-AOC)、8-异前列腺素F2α(8-iso-PGF2α)及脂质过氧化产物(LPO)水平变化及意义。方法采用酶联免疫吸附法检测33名井上健康工人(对照组),40名井下健康接尘矿工(接尘组)及91例壹期CWP患者(CWP组)血清中HO-1、Nrf2和8-iso-PGF2α水平;化学比色法检测血清T-AOC和LPO水平。结果与对照组比较,接尘组Nrf2、T-AOC和LPO水平升高,CWP组LPO水平升高,差异均有统计学意义(P<0.05,P<0.01),CWP组T-AOC水平低于接尘组(P<0.01),CWP组饮酒患者血清中HO-1、Nrf2和8-iso-PGF2α水平低于非饮酒者(P<0.05)。CWP组HO-1和Nrf2、8-iso-PGF2α,Nrf2和8-iso-PGF2α水平呈明显正相关(rs值分别为0.840、0.882、0.885,P<0.01)。结论氧化应激反应与壹期CWP发生发展有关;饮酒可影响CWP患者氧化应激水平;检测TAOC水平有助于CWP的早期筛检。
Objective To investigate the changes of serum oxidative stress indexes such as heme oxygenase-1 (HO-1), nuclear factor E2 (Nrf2), total antioxidant capacity (T-AOC) Changes and significance of iso prostaglandin F2α (8-iso-PGF2α) and lipid peroxidation product (LPO) Methods Serum levels of HO-1, Nrf2 and 8 in 33 healthy workers (control group), 40 underground miners (dust group) and 91 primary CWP patients (CWP group) were detected by enzyme-linked immunosorbent assay -iso-PGF2α levels; serum T-AOC and LPO levels were detected by chemical colorimetry. Results Compared with the control group, the levels of Nrf2, T-AOC and LPO in the dust-exposed group increased and the levels of LPO increased in the CWP group (P <0.05, P <0.01). The levels of T-AOC in the CWP group were lower The levels of HO-1, Nrf2 and 8-iso-PGF2α in CWP group were lower than those in non-drinkers group (P <0.05). The levels of HO-1 and Nrf2, 8-iso-PGF2α, Nrf2 and 8-iso-PGF2αwere positively correlated in CWP group (rs = 0.840,0.882,0.885, P <0.01). Conclusions Oxidative stress is associated with the occurrence and development of one-stage CWP. Alcohol consumption can affect the level of oxidative stress in CWP patients. Detecting TAOC levels is helpful for the early screening of CWP.