【摘 要】
：
根据6个春麦品种及4个冬麦品种的试验结果,提高诱导小麦未成熟胚绿苗率的关键是:(1)掌握最适宜的接种时间,并要求幼胚的盾状组织朝上放置。(2)诱导愈伤组织的培养基(A)的最佳
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根据6个春麦品种及4个冬麦品种的试验结果,提高诱导小麦未成熟胚绿苗率的关键是:(1)掌握最适宜的接种时间,并要求幼胚的盾状组织朝上放置。(2)诱导愈伤组织的培养基(A)的最佳配方为MS培养基,3％蔗糖,245-T 2mg/J,IAA0.4mg/J,激动素0.2 mg/J,琼脂0.75％,pH5.7。主要改动是以245-T代替2,4-D,并提高IAA的浓度。新方法的愈伤组织诱导率高达85—100％,以幼胚为计算基础的绿苗诱导率为84—99％,每个萌动的幼胚一般可产生健壮的绿苗16—20株。
Based on the testing results of 6 spring wheat varieties and 4 winter wheat varieties, the key to improve the rate of immature embryo-induced green shoots is: (1) to master the most appropriate time for vaccination and to require the immature embryo shield tissue to be placed upwards. (2) The best medium for inducing callus (A) was MS medium, 3% sucrose, 245-T 2mg / J, IAA 0.4mg / J, kinetin 0.2mg / J, agar 0.75% pH5.7. The main change is the 245-T instead of 2,4-D, and increase the concentration of IAA. The callus induction rate of the new method is as high as 85-100%, and the green shoot induction rate based on the immature embryo is 84-99%. Each young embryo generally produces 16-20 strong green shoots.
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